CGSC Strain#: 4136
Strain Designation: AB4136      Source of Strain: B.B. Magee
Sex: F- Chromosomal Markers: araC14, lacY1, tsx-57, glnX44(AS), galK2(Oc), metC56, xyl-7 or xylA5, thiE1, valS10(ts), tfr-5
Strain Comments:

• Parent of this strain (AB4119) is reported to be P1 resistant.
• This strain stops growth immediately at 37 degrees C.
• glnX44(AS)-- Mutation is a C to T transition at nucleotide 34 (3rd position of the anticodon)
• galK2(Oc)-- is a GAA(glu) to TAA(ochre) mutation, residue 134. Oller et al.1993. Apparently non-suppressible, since all revertants found were at the ochre site.
• galK2(Oc) was formerly called gal₂
• glnX44(AS) was formerly called glnV44
• glnX44(AS) was formerly called su⁺_II
• glnX44(AS) was formerly called supE44
• thiE1 was formerly called thi-1
• AS = amber(UAG) suppressor
• Oc = ochre(UAA) mutation
• ts = Temperature Sensitive

References:

• Korner, A, BB Magee, B Liska, KB Low, EA Adelberg, D Soll 1974. Isolation and partial characterization of a temperature-sensitive Escherichia coli mutant with altered glutaminyl-transfer ribonucleic acid synthetase. J. Bacteriol. 120(1):154-8.