CGSC Strain#: 4131
Strain Designation: AB4131      Source of Strain: E.A. Adelberg
Sex: F- Chromosomal Markers: araC14, lacY1, glnX44(AS), galK2(Oc), metC56, xyl-7 or xylA5, lldD1, thiE1, valS7(ts), tfr-5
Strain Comments:

• glnX44(AS)-- Mutation is a C to T transition at nucleotide 34 (3rd position of the anticodon)
• galK2(Oc)-- is a GAA(glu) to TAA(ochre) mutation, residue 134. Oller et al.1993. Apparently non-suppressible, since all revertants found were at the ochre site.
• valS7(ts)-- is a temperature-sensitive mutation. Growth stops at 35 degrees C.
• galK2(Oc) was formerly called gal₂
• glnX44(AS) was formerly called glnV44
• glnX44(AS) was formerly called su⁺_II
• glnX44(AS) was formerly called supE44
• thiE1 was formerly called thi-1
• AS = amber(UAG) suppressor
• Oc = ochre(UAA) mutation
• ts = Temperature Sensitive

References:

• Baer, M.F., K.B. Low, D. Soll 1979. Regulation of the biosynthesis of aminoacyl-transfer ribonucleic acid synthetases and of transfer-ribonucleic acid in Escherichia coli. V. Mutants with increased levels of valyl-transfer ribonucleic acid synthetase.