CGSC Strain#: 4010
Strain Designation: AB4010      Source of Strain: M.G. Marinus
Sex: F- Chromosomal Markers: thr-1, leuB6(Am), fhuA21, lacY1, tsm-7(DNA(ts)), glnX44(AS), λ^-, thyA6, rpsL67(strR), thiE1, deoC1, spc-9
Strain Comments:

• leuB6(Am)-- Suppressed by serU suppressor (supH)
• tsm-7(DNA(ts))-- = DNA_TS -7a of Bonhoeffer. Maps between min.91-97, so probably in dnaB.
• glnX44(AS)-- Mutation is a C to T transition at nucleotide 34 (3rd position of the anticodon)
• thyA6-- Strains with this marker must have their medium supplemented with thymine (25ug/ml). This is true even for rich medium such as LB. Otherwise thymine starvation can lead to thymineless death.
• thyA6-- : Even with Luria or other complete media, supplement with thymine.
• glnX44(AS) was formerly called su⁺_II
• glnX44(AS) was formerly called supE44
• glnX44(AS) was formerly called glnV44
• thiE1 was formerly called thi-1
• Am = amber(UAG) mutation
• AS = amber(UAG) suppressor
• DNA(ts) = DNA temperature-sensitive
• strR = streptomycin resist.

References:

• Marinus, MG, EA Adelberg 1970. Vegetative Replication and Transfer Replication of Deoxyribonucleic Acid in Temperature-Sensitive Mutants of Escherichia coli K-12. 104(3):1266-1272.