CGSC Strain#: 3521
Strain Designation: AB3521      Source of Strain: J.A. Wechsler
Other Designations: JW38
Sex: F' Episome/Plasmid: F2016-1
   Plasmid Markers/Mutations: ilvD188(Oc), ilvE , ilvA
Chromosomal Markers: araBAD-9, fhuA1, Δ(gpt-proA)62, lacY1 or lacZ4, tsx-3, galK2(Oc), trpE3(Oc), hisG4(Oc), relA1, rpsL8 or rplL9(L?), malT1(λ^R), mtl-1, ilvD188(Oc), thiE1, sup-48
Strain Comments:

• galK2(Oc)-- is a GAA(glu) to TAA(ochre) mutation, residue 134. Oller et al.1993. Apparently non-suppressible, since all revertants found were at the ochre site.
• sup-48-- weak amber suppressor.
• Δ(gpt-proA)62 was formerly called proA2
• galK2(Oc) was formerly called gal₂
• thiE1 was formerly called thi-1
• LamR = Lambda-resistant
• Oc = ochre(UAA) mutation
• strR = streptomycin resist.
• Oc = ochre(UAA) mutation

References:

• Wechsler, J.A. 1969. Antipolarity in the ilv operon of Escherichia coli K-12. J.Bacteriol. 98:1179-1194