CGSC Strain#: 3048
Strain Designation: AB3048, LAM-      Source of Strain: P. Howard-Flanders
Sex: F- Chromosomal Markers: glnX44(AS), λ^-, uvrB5, thyA34, endA1, xylA5, thiE1, deo-220
Strain Comments:

• This strain may be P1 lysogen. The deo marker is either deoB or deoC.
• glnX44(AS)-- Mutation is a C to T transition at nucleotide 34 (3rd position of the anticodon)
• thyA34-- Strains with this marker must have their medium supplemented with thymine (25ug/ml). This is true even for rich medium such as LB. Otherwise thymine starvation can lead to thymineless death.
• endA1-- G to A transition mutation resulting in E208K amino acid substitution.
• glnX44(AS) was formerly called glnV44
• glnX44(AS) was formerly called su⁺_II
• glnX44(AS) was formerly called supE44
• thiE1 was formerly called thi-1
• = good miniprep dsDNA quality
• AS = amber(UAG) suppressor

References:

• Howard-Flanders, P. 1968. Cold Spr.Harb.Symp.Quant.Biol. 33:195-0