CGSC Strain#: 13589
Strain Designation: DH5α/pBAD-A      Source of Strain:
Other Designations: DH5alpha/pBAD-A
Other Designations: pBAD-A
Sex: F- Episome/Plasmid: pBAD-A
   Plasmid Markers/Mutations: ori_p15A, araC , bla , clsA
   Plasmid Comment: pBAD30 with clsA inserted between XbaI and SphI
Chromosomal Markers: Δ(argF-lac)169, φ80dlacZ58(M15), ΔphoA8, glnX44(AS), λ^-, deoR481, rfbC1, gyrA96(NalR), recA1, endA1, thiE1, hsdR17
Strain Comments:

• DH5 is a non-mutagenized derivative of DH1, which transforms more efficiently due to a deoR mutation.
• Δ(argF-lac)169-- extends from mmuP through orfs preceding argF, through lac to mhpD, literally Δ(mmuP-mhpD)169. (Peters et al. 2003 JB 185:2017)
• Δ(argF-lac)169-- from strain Hfr3000 U169 was initially called ΔlacU169 and described as a lacZY mutation until found to include argF and lacI.
• glnX44(AS)-- Mutation is a C to T transition at nucleotide 34 (3rd position of the anticodon)
• deoR481-- A selectable phenotype of deoR mutants includes the ability to grow preferentially on minimal medium with inosine as the sole carbon source.
• gyrA96(NalR)-- GAC to AAC at codon 87
• gyrA96(NalR)-- resistant to 200ug/ml nalidixic acid
• recA1-- : Missense mutation, altered isoelectric point. Sequenced: G to A for Nuc. 720 (Gly 160 Asp).
• endA1-- G to A transition mutation resulting in E208K amino acid substitution.
• glnX44(AS) was formerly called glnV44
• glnX44(AS) was formerly called su⁺_II
• glnX44(AS) was formerly called supE44
• gyrA96(NalR) was formerly called
• rfbC1 was formerly called rfbD1
• thiE1 was formerly called thi-1
• = good miniprep dsDNA quality
• AS = amber(UAG) suppressor
• NalR = Nalidix.acidResist.

References:

• Tan, BK, M Bogdanov, J Zhao, W Dowhan, CR Raetz, Z Guan 2012. Discovery of a cardiolipin synthase utilizing phosphatidylethanolamine and phosphatidylglycerol as substrates. Proc. Natl. Acad. Sci. U.S.A. 109(41):16504-9.