CGSC Strain#: 13639
Strain Designation: pLC6-32      Source of Strain:
Sex: F+ Episome/Plasmid: pLC6-32A
   Plasmid Markers/Mutations: araC14, thr-1, leuB6(Am), lacY1, glnX44(AS), galK2(Oc), galT22, λ^-, ΔtrpE63, recA56, xylA5, mtl-1, thiE1, glnD , garB , mrcB , fhuA , fhuB , fhuC , fhuD , cdsA , rpsB , tsf , dgt , hemL , dapC , dapD , hemF
   Plasmid Comment: Hybridizes with Kohara clones no. 116, 117, 118, 119, 120, 121 and also no. 618 (mixed clone). Carries hemF ca. 55 minutes. See also pLC6-32B and pLC6-32C.
Chromosomal Markers: thr-1, araC14, leuB6(Am), lacY1, glnX44(AS), galK2(Oc), galT22, λ^-, ΔtrpE63, recA56, xylA5, mtl-1, thiE1
Strain Comments:

• leuB6(Am)-- Suppressed by serU suppressor (supH)
• glnX44(AS)-- Mutation is a C to T transition at nucleotide 34 (3rd position of the anticodon)
• galK2(Oc)-- is a GAA(glu) to TAA(ochre) mutation, residue 134. Oller et al.1993. Apparently non-suppressible, since all revertants found were at the ochre site.
• recA56-- Amino acid substitution Arg 60 Cys
• galK2(Oc) was formerly called gal₂
• glnX44(AS) was formerly called su⁺_II
• glnX44(AS) was formerly called supE44
• glnX44(AS) was formerly called glnV44
• thiE1 was formerly called thi-1
• ΔtrpE63 was formerly called DE(trpE5)
• Am = amber(UAG) mutation
• AS = amber(UAG) suppressor
• Oc = ochre(UAA) mutation
• Am = amber(UAG) mutation
• AS = amber(UAG) suppressor
• Oc = ochre(UAA) mutation

References:

• Clarke, L, J Carbon 1976. A colony bank containing synthetic Col El hybrid plasmids representative of the entire E. coli genome. Cell 9(1):91-9.
• Garcia, G.M., M. Federici, S.G. Rhee, M.A. Berberich 1980. Glutamine synthetase cascade: enrichment of uridylytransferase in Escherichia coli carrying hybrid ColE1 plasmids. Arch.Biochem. 203:181-189
• Neidhardt, F.C., V. Vaughn, T.A. Phillips, P.L. Bloch 1983. Gene-protein index of Escherichia coli K-12. Microbiol.Rev. 47:231-284
• Ichikawa, S., M. Ryoji, Z. Siegfried, A. Kaji 1989. Localization of the ribosome-releasing factor gene in the Escherichia coli chromosome. J.Bacteriol. 171:3689-3695
• Nishimura, A., K. Akiyama, Y. Kohara, K. Horiuchi 1992. Correlation of a subset of the pLC plasmids to the physical map of Escherichia coli K-12. Microbiol.Rev. 56:137-151