CGSC Strain#: 2383
Strain Designation: AB2383      Source of Strain: P. Howard-Flanders
Other Designations: P4X, Thy^- Hfr
Sex: Hfr    Point of Origin#: 3   Map Position: 6.80   Direction of Marker Transfer: Counterclockwise
Chromosomal Markers: relA1, thyA2, metB1
Strain Comments:

• This strain was made in the Zoology Department at Columbia University, but we do not know its original designation.
• thyA2-- Strains with this marker must have their medium supplemented with thymine (25ug/ml). This is true even for rich medium such as LB. Otherwise thymine starvation can lead to thymineless death.

References:

• Howard-Flanders, P., R.P. Boyce, L. Theriot 1966. Three loci in Escherichia coli K-12 that control the excision of pyrimidine dimers and certain other mutagen products from DNA. Genetics 53:1119-1136
• Emmerson, P.T. 1968. Recombination deficient mutants of Escherichia coli K12 that map between thyA and argA. Genetics 60:19-30