CGSC Strain#: 1035
Strain Designation: AB1035      
Source of Strain: E.A. Adelberg
Sex: F' 
Episome/Plasmid: F14-21
   Plasmid Markers/Mutations: ilvP-271 Chromosomal Markers: lacY1 or lacZ4, 
tsx-6, 
galK2(Oc)
, 
xylA5, 
ilvC7, 
argE3(Oc)
, 
thiE1, 
tfr-3Strain Comments: - galK2(Oc)-- is a GAA(glu) to TAA(ochre) mutation, residue 134. Oller et al.1993. Apparently non-suppressible, since all revertants found were at the ochre site.                                                                                                          
- galK2(Oc) was formerly called gal2
- thiE1 was formerly called thi-1
- Oc = ochre(UAA) mutation
References:- Ramakrishnan, T., E.A. Adelberg 1964. Regulatory mechanisms in the biosynthesis of isoleucine and valine. I. Genetic derepression of enzyme formation. J.Bacteriol. 87:566-573
- Ramakrishnan, T., E.A. Adelberg 1965. Regulatory mechanisms in the biosynthesis of isoleucine and valine. II. Identification of two operator genes. J.Bacteriol. 89:654-660