CGSC Strain#: 1001
Strain Designation: AB1001      Source of Strain: T. Ramakrishnan & Adelberg
Sex: F' Episome/Plasmid: F14-6
   Plasmid Markers/Mutations: ilvO-266
   Plasmid Comment: carries {\it ilvE266} mutation.
Chromosomal Markers: Δ(gpt-proA)62, lacY1, glnX44(AS), galK2(Oc), hisG4(Oc), rpsL8, Δ(ilvE-argH)2, thiE1, tfr-3
Strain Comments:

• This strain is valine-resistant.
• glnX44(AS)-- Mutation is a C to T transition at nucleotide 34 (3rd position of the anticodon)
• galK2(Oc)-- is a GAA(glu) to TAA(ochre) mutation, residue 134. Oller et al.1993. Apparently non-suppressible, since all revertants found were at the ochre site.
• Δ(ilvE-argH)2-- is the deletion corresponding to the insert in F14.
• Δ(gpt-proA)62 was formerly called proA2
• galK2(Oc) was formerly called gal₂
• glnX44(AS) was formerly called glnV44
• glnX44(AS) was formerly called su⁺_II
• glnX44(AS) was formerly called supE44
• thiE1 was formerly called thi-1
• AS = amber(UAG) suppressor
• Oc = ochre(UAA) mutation

References:

• Ramakrishnan, T., E.A. Adelberg 1964. Regulatory mechanisms in the biosynthesis of isoleucine and valine. I. Genetic derepression of enzyme formation. J.Bacteriol. 87:566-573