CGSC Strain#: 6943
Strain Designation: LJ111      Source of Strain: W.D. Nunn
Sex: F- Chromosomal Markers: lacY1 or Δ(codB-lacI)3, glnX44(AS), galK2(Oc), galT22, λ^-, tyrT58(AS), Δ(atoC-atoB)540, metB1, hsdR514, trpR55
Strain Comments:

• glnX44(AS)-- Mutation is a C to T transition at nucleotide 34 (3rd position of the anticodon)
• galK2(Oc)-- is a GAA(glu) to TAA(ochre) mutation, residue 134. Oller et al.1993. Apparently non-suppressible, since all revertants found were at the ochre site.
• trpR55-- is trpR2 of Yanofsky. It is a frameshift mutation. Trp levels are indistinguishable from those in trpR deletions or amber mutations (no functional repressor).
• galK2(Oc) was formerly called gal₂
• glnX44(AS) was formerly called su⁺_II
• glnX44(AS) was formerly called supE44
• glnX44(AS) was formerly called glnV44
• tyrT58(AS) was formerly called supF58
• AS = amber(UAG) suppressor
• Oc = ochre(UAA) mutation

References:

• Jenkins, L.S., W.D. Nunn 1987. Genetic and molecular characterization of the genes involved in short-chain fatty acid degradation in Escherichia coli: the ato system. J.Bacteriol. 169:42-52