CGSC Strain#: 12547
Strain Designation: CJ815      Source of Strain: C.M. Joyce
Other Designations: CJ815/pEP402
Other Designations: XL1-Blue/pEP402
Sex: F' Episome/Plasmid: F128-Tn10-11
   Plasmid Markers/Mutations: lacIp-4000(lacI^Q), ΔlacZ58(M15), F::Tn10-11, proA , proB , Tn10-11 , lac
Episome/Plasmid: pEP402
   Plasmid Markers/Mutations:
   Plasmid Comment: A full description of this plasmid is not yet in our database
Chromosomal Markers: Δ(gpt-lac)0, glnX44(AS), λ^-, rfbC1, gyrA96(NalR), recA1, relA1, endA1, thiE1, hsdR17
Strain Comments:

• glnX44(AS)-- Mutation is a C to T transition at nucleotide 34 (3rd position of the anticodon)
• gyrA96(NalR)-- GAC to AAC at codon 87
• gyrA96(NalR)-- resistant to 200ug/ml nalidixic acid
• recA1-- : Missense mutation, altered isoelectric point. Sequenced: G to A for Nuc. 720 (Gly 160 Asp).
• endA1-- G to A transition mutation resulting in E208K amino acid substitution.
• glnX44(AS) was formerly called glnV44
• glnX44(AS) was formerly called su⁺_II
• glnX44(AS) was formerly called supE44
• gyrA96(NalR) was formerly called
• rfbC1 was formerly called rfbD1
• thiE1 was formerly called thi-1
• = good miniprep dsDNA quality
• AS = amber(UAG) suppressor
• NalR = Nalidix.acidResist.
• = tetracycline resistant
• M15 = alpha-complemented allele

References: