CGSC Strain#: 6575
Strain Designation: AE84064      Source of Strain: J.J. Anderson
Sex: F- Chromosomal Markers: fhuA2::IS2, lacY1, glnX44(AS), lky-501::Mu cts, gal-6, λ^-, lrp-1, serC53, trp-31, hisG1, gyrA260(NalR), thyA746, argG6, rpsL104, malT1(λ^R), xyl-7, mtlA2
Strain Comments:

• glnX44(AS)-- Mutation is a C to T transition at nucleotide 34 (3rd position of the anticodon)
• hisG1-- This mutation has been reported as either a frameshift or a 36 nt deletion
• thyA746-- Strains with this marker must have their medium supplemented with thymine (25ug/ml). This is true even for rich medium such as LB. Otherwise thymine starvation can lead to thymineless death.
• fhuA2::IS2 was formerly called fhuA2
• glnX44(AS) was formerly called su⁺_II
• glnX44(AS) was formerly called supE44
• glnX44(AS) was formerly called glnV44
• lrp-1 was formerly called livR1
• serC53 was formerly called serC3
• serC53 was formerly called pdxC3
• xyl-7 was formerly called xylA7
• = strep-resistant
• ::IS2 = Insertion of IS2
• AS = amber(UAG) suppressor
• LamR = Lambda-resistant
• NalR = Nalidix.acidResist.

References:

• Anderson, J.J., J.M. Wilson, D.L. Oxender 1979. Defective transport and other phenotypes of a periplastic "leaky" mutant of Escherichia coli K-12. J.Bacteriol. 140:351-358