CGSC Strain#: 6022
Strain Designation: SK2267      Source of Strain: S.R. Kushner
Sex: F- Chromosomal Markers: lacZ4 or lac-61, glnX44(AS), gal-44, λ^-, sbcB15, recA1, endA1, rfa-([C]), thiE1, hsdR4, ton-58
Strain Comments:

• 1.We do not know of a reference to SK2267. The following is a reference to SK1590, the ton⁺ parent of SK1592:
• 2.Kushner, S 1978 An improved method for transformation of Escherichia coli with ColE1 derived plasmids, p. 17-23. In H.W. Boyer and S. Nicosia (ed), Genetic Engineering. Elsevier North
• 3.Holland Biomedical Press.
• The strain is φX^S. Other parts of the chromosome could have come from E. coli C.
• glnX44(AS)-- Mutation is a C to T transition at nucleotide 34 (3rd position of the anticodon)
• recA1-- : Missense mutation, altered isoelectric point. Sequenced: G to A for Nuc. 720 (Gly 160 Asp).
• endA1-- G to A transition mutation resulting in E208K amino acid substitution.
• rfa-([C])-- comes from E. coli C.
• glnX44(AS) was formerly called su⁺_II
• glnX44(AS) was formerly called supE44
• glnX44(AS) was formerly called glnV44
• thiE1 was formerly called thi-1
• = good miniprep dsDNA quality
• [C] = from E. coli C.
• AS = amber(UAG) suppressor

References: