CGSC Strain#: 12445
Strain Designation: N6197      Source of Strain: R.G. Lloyd
Other Designations: AM1675
Sex: F- Chromosomal Markers: λ^-, ΔrecB1910::dhfr, rph-1
Strain Comments:

• ΔrecB1910::dhfr-- The deletion starts 63bp into recB and extends to 147bp from the end of the gene. The deletion/insertion joints are as follows with chromosomal sequence in uppercase and dhfr cassette in lowercase: CGCCTGATT/tgcaagcag..........ggatgctga/ACCACTTTG.
• rph-1-- is a 1 bp deletion that results in frameshift over last 15 codons and has polar effect on pyrE leading to suboptimal pyrimidine levels on minimal medium.(Jensen 1993 JBact.175:3401)

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