CGSC Strain#: 12444
Strain Designation: N6159      Source of Strain: R.G. Lloyd
Other Designations: AM1672
Sex: F- Chromosomal Markers: λ^-, ΔrecD1911::dhfr, rph-1
Strain Comments:

• ΔrecD1911::dhfr-- The deletion starts 49bp into recD and extends to 81bp from the end of the gene. The deletion/insertion joints are as follows with chromosomal sequence in uppercase and dhfr cassette in lowercase: ACAGCTACG/tgcaagcag..........ggatgctga/TGAGCGCAT.
• rph-1-- is a 1 bp deletion that results in frameshift over last 15 codons and has polar effect on pyrE leading to suboptimal pyrimidine levels on minimal medium.(Jensen 1993 JBact.175:3401)

References:

• Mahdi, AA, C Buckman, L Harris, RG Lloyd 2006. Rep and PriA helicase activities prevent RecA from provoking unnecessary recombination during replication fork repair. Genes Dev. 20(15):2135-47.