CGSC Strain#: 12491
Strain Designation: N6052      Source of Strain: R.G. Lloyd
Other Designations: AM1655
Sex: F- Chromosomal Markers: λ^-, rph-1, ΔrecG1919::apra
Strain Comments:

• rph-1-- is a 1 bp deletion that results in frameshift over last 15 codons and has polar effect on pyrE leading to suboptimal pyrimidine levels on minimal medium.(Jensen 1993 JBact.175:3401)
• apra = Apramycin resistant

References:

• Mahdi, AA, C Buckman, L Harris, RG Lloyd 2006. Rep and PriA helicase activities prevent RecA from provoking unnecessary recombination during replication fork repair. Genes Dev. 20(15):2135-47.
• Rudolph, CJ, AA Mahdi, AL Upton, RG Lloyd 2010. RecG protein and single-strand DNA exonucleases avoid cell lethality associated with PriA helicase activity in Escherichia coli. Genetics 186(2):473-92.
• Zhang, J, AA Mahdi, GS Briggs, RG Lloyd 2010. Promoting and avoiding recombination: contrasting activities of the Escherichia coli RuvABC Holliday junction resolvase and RecG DNA translocase. Genetics 185(1):23-37.