CGSC Strain#: 12494
Strain Designation: N6390      Source of Strain: R.G. Lloyd
Other Designations: AM1716
Sex: F- Chromosomal Markers: λ^-, Δ(recD-recC)802(::apra), rph-1
Strain Comments:

• For selection in transductions, use apramycin at a final concentration of no more than 30ug/ml in LB agar
• Δ(recD-recC)802(::apra)-- The deletion/insertion joints are as follows with chromosomal sequence in uppercase and apramycin:FRT cassette in lowercase: GAAGCGTTG/tcatgtgca..........ggaacttcc/CGATGAGCG.
• rph-1-- is a 1 bp deletion that results in frameshift over last 15 codons and has polar effect on pyrE leading to suboptimal pyrimidine levels on minimal medium.(Jensen 1993 JBact.175:3401)
• apra = Apramycin resistant

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