CGSC Strain#: 12433
Strain Designation: N7475      Source of Strain: R.G. Lloyd
Other Designations: AM2006
Sex: F- Chromosomal Markers: λ^-, Δ(ruvB-ruvC)767(::kan), rph-1
Strain Comments:

• Δ(ruvB-ruvC)767(::kan)-- The deletion starts 39bp into ruvC and extends to 39bp from the end of ruvB. The deletion/insertion joints are as follows with chromosomal sequence in uppercase and kan cassette in lowercase: TCGCGCGTG/ccatcatcg..........cgtggcggg/TGGAATCAC.
• rph-1-- is a 1 bp deletion that results in frameshift over last 15 codons and has polar effect on pyrE leading to suboptimal pyrimidine levels on minimal medium.(Jensen 1993 JBact.175:3401)
• = kanamycin resistant

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