CGSC Strain#: 12416
Strain Designation: STL14081      Source of Strain: S.T. Lovett
Sex: F- Chromosomal Markers: lacZ4812, mhpC281::Tn10, λ^-, ΔmutS738::kan, rph-1
Strain Comments:

• lacZ4812-- is a 2 basepair substitution (GAA to AAG) in the Glu-461 codon resulting in a Lys codon.
• mhpC281::Tn10-- cotransduces 80% with lac.
• ΔmutS738::kan-- The primers used to make this knockout were AAAAACCATCACACCCCATTTAATATCAGGGAACCGGACATAACCCCATGATTCCGGGGATCCGTCGACC and CGATAGCAAAAGACTATCGGGAATTGTTATTACACCAGGCTCTTCAAGCGTGTAGGCTGGAGCTGCTTCG the bolded sequence matches the pKD13 template
• rph-1-- is a 1 bp deletion that results in frameshift over last 15 codons and has polar effect on pyrE leading to suboptimal pyrimidine levels on minimal medium.(Jensen 1993 JBact.175:3401)
• lacZ4812 was formerly called lacZ(A1384G)
• mhpC281::Tn10 was formerly called zah281::Tn10
• = tetracycline resistant
• = kanamycin resistant

References:

• Seier, T, DR Padgett, G Zilberberg, VA Sutera, N Toha, ST Lovett 2011. Insights into mutagenesis using Escherichia coli chromosomal lacZ strains that enable detection of a wide spectrum of mutational events. Genetics 188(2):247-62.