CGSC Strain#: 12417
Strain Designation: STL14097      Source of Strain: S.T. Lovett
Sex: F- Chromosomal Markers: lacZ4814, mhpC281::Tn10, λ^-, ΔmutS738::kan, rph-1
Strain Comments:

• lacZ4814-- is a compound allele replacing GGC GGC GGA with GGG GGGC GGA starting at base 1465 in the lacZ sequence. This results in one synonymous substitution and a single basepair insertion of a G residue causing a frameshift mutation at base 1470.
• mhpC281::Tn10-- cotransduces 80% with lac.
• ΔmutS738::kan-- The primers used to make this knockout were AAAAACCATCACACCCCATTTAATATCAGGGAACCGGACATAACCCCATGATTCCGGGGATCCGTCGACC and CGATAGCAAAAGACTATCGGGAATTGTTATTACACCAGGCTCTTCAAGCGTGTAGGCTGGAGCTGCTTCG the bolded sequence matches the pKD13 template
• rph-1-- is a 1 bp deletion that results in frameshift over last 15 codons and has polar effect on pyrE leading to suboptimal pyrimidine levels on minimal medium.(Jensen 1993 JBact.175:3401)
• lacZ4814 was formerly called lacZ(-1G1474)
• mhpC281::Tn10 was formerly called zah281::Tn10
• = tetracycline resistant
• = kanamycin resistant

References:

• Seier, T, DR Padgett, G Zilberberg, VA Sutera, N Toha, ST Lovett 2011. Insights into mutagenesis using Escherichia coli chromosomal lacZ strains that enable detection of a wide spectrum of mutational events. Genetics 188(2):247-62.