CGSC Strain#: 6438
Strain Designation: T31-4      Source of Strain: Lawther & Hatfield
Sex: F- Chromosomal Markers: lacY1 or lacZ4, glnX44(AS), trpA9761(Am), trpE9829(Am), dsdA1, rpsL8 or rplL9(L?) or rpsL14, malT1(λ^R), xyl-7, mtlA2, ilvD188(Oc), thiE1, tsx-23 or tsx-25
Strain Comments:

• The trp alleles are amber mutations: ilvD188 is ochre. This strain is isogenic to T31-H except for the purF-hisT region, which was transduced in from different backgrounds.
• glnX44(AS)-- Mutation is a C to T transition at nucleotide 34 (3rd position of the anticodon)
• dsdA1-- is called dsdA7 by McFall (1967 Genetics 55:91).
• glnX44(AS) was formerly called su⁺_II
• glnX44(AS) was formerly called supE44
• glnX44(AS) was formerly called glnV44
• thiE1 was formerly called thi-1
• trpA9761(Am) was formerly called trp-40
• trpA9761(Am) was formerly called trp-47
• xyl-7 was formerly called xylA7
• Am = amber(UAG) mutation
• AS = amber(UAG) suppressor
• LamR = Lambda-resistant
• Oc = ochre(UAA) mutation
• strR = streptomycin resist.

References:

• Bruni, C.B., V. Colantuoni, L. Sbordone, R. Cortese, F. Blasi 1977. Biochemical and regulatory properties of Escherichia coli K-12 hisT mutants. J.Bacteriol. 130:4-10
• Lawther, R.P., G.W. Hatfield 1977. Biochemical characterization of an Escherichia coli hisT strain. J.Bacteriol. 130:552-557