CGSC Strain#: 5913
Strain Designation: W5449      Source of Strain: J. Lederberg
Sex: F- Chromosomal Markers: araC14, leuB6(Am), Δ(gpt-proA)62, lacY1, tsx-33, glnX44(AS), galK2(Oc), λ^-, tonB56, trpB9579(Oc), Rac-0, sbcB15, hisG4(Oc), rfbC1, recB21, recC22, rpsL31(strR), kdgK51, xylA5, mtl-1, argE3(Oc), thiE1, hsdR18
Strain Comments:

• This strain is inexplicably Trp⁺ and φ 80^S
• leuB6(Am)-- Suppressed by serU suppressor (supH)
• glnX44(AS)-- Mutation is a C to T transition at nucleotide 34 (3rd position of the anticodon)
• galK2(Oc)-- is a GAA(glu) to TAA(ochre) mutation, residue 134. Oller et al.1993. Apparently non-suppressible, since all revertants found were at the ochre site.
• Δ(gpt-proA)62 was formerly called proA2
• galK2(Oc) was formerly called gal₂
• glnX44(AS) was formerly called su⁺_II
• glnX44(AS) was formerly called supE44
• glnX44(AS) was formerly called glnV44
• rfbC1 was formerly called rfbD1
• thiE1 was formerly called thi-1
• Am = amber(UAG) mutation
• AS = amber(UAG) suppressor
• Oc = ochre(UAA) mutation
• strR = streptomycin resist.

References:

• Sgaramella, V, SD Ehrlich, H Bursztyn, J Lederberg 1976. Enhancement of transfecting activity of bacteriophage P22 DNA upon exonucleolytic erosion. J. Mol. Biol. 105(4):587-602.