CGSC Strain#: 12137
Strain Designation: JM101     
Source of Strain: E.A. Raleigh
Sex: F'
Episome/Plasmid: F128-x
Plasmid Markers/Mutations: lacIp-4000(lacIQ),
ΔlacZ58(M15)
,
traD36,
proA ,
proB Plasmid Comment: an F128-like F' found in Messing strains and their derivatives
Chromosomal Markers: Δ(gpt-lac)0,
glnX44(AS)
,
thiE1Strain Comments: - Deposited by New England Biolabs because they were no longer going to distribute this strain
- glnX44(AS)-- Mutation is a C to T transition at nucleotide 34 (3rd position of the anticodon)
- glnX44(AS) was formerly called supE44
- glnX44(AS) was formerly called glnV44
- glnX44(AS) was formerly called su+II
- thiE1 was formerly called thi-1
- AS = amber(UAG) suppressor
- M15 = alpha-complemented allele
References: - Messing, J, R Crea, PH Seeburg 1981. A system for shotgun DNA sequencing. Nucleic Acids Res. 9(2):309-21.