CGSC Strain#: 12326
Strain Designation: DH5α/pEXT21      Source of Strain: T. Linn
Other Designations: DH5alpha/pEXT21
Other Designations: pEXT21
Sex: F- Episome/Plasmid: pEXT21
   Plasmid Markers/Mutations: aadA(strR,SpcR), lacIp-4000(lacI^Q)
Chromosomal Markers: Δ(argF-lac)169, φ80dlacZ58(M15), ΔphoA8, glnX44(AS), λ^-, deoR481, rfbC1, gyrA96(NalR), recA1, endA1, thiE1, hsdR17
Strain Comments:

• Δ(argF-lac)169-- from strain Hfr3000 U169 was initially called ΔlacU169 and described as a lacZY mutation until found to include argF and lacI.
• Δ(argF-lac)169-- extends from mmuP through orfs preceding argF, through lac to mhpD, literally Δ(mmuP-mhpD)169. (Peters et al. 2003 JB 185:2017)
• glnX44(AS)-- Mutation is a C to T transition at nucleotide 34 (3rd position of the anticodon)
• deoR481-- A selectable phenotype of deoR mutants includes the ability to grow preferentially on minimal medium with inosine as the sole carbon source.
• gyrA96(NalR)-- GAC to AAC at codon 87
• gyrA96(NalR)-- resistant to 200ug/ml nalidixic acid
• recA1-- : Missense mutation, altered isoelectric point. Sequenced: G to A for Nuc. 720 (Gly 160 Asp).
• endA1-- G to A transition mutation resulting in E208K amino acid substitution.
• glnX44(AS) was formerly called glnV44
• glnX44(AS) was formerly called su⁺_II
• glnX44(AS) was formerly called supE44
• gyrA96(NalR) was formerly called
• rfbC1 was formerly called rfbD1
• thiE1 was formerly called thi-1
• = good miniprep dsDNA quality
• AS = amber(UAG) suppressor
• NalR = Nalidix.acidResist.
• SpcR = spectinomycin resistant
• strR = streptomycin resist.

References:

• Dykxhoorn, DM, R St Pierre, T Linn 1996. A set of compatible tac promoter expression vectors. Gene 177(1-2):133-6.