CGSC Strain#: 5732
Strain Designation: W945T1-2      Source of Strain: Courtright & Henning
Sex: F- Chromosomal Markers: thr-1, araC14, leuB6(Am), lacY1, galK2(Oc), trp-59, mdh-2, rpsL(strR), xylA5, mtl-1, thiE1
Strain Comments:

• The derivation of this strain is not known completely. We have not been able to assign an allele number to the rpsL mutation.
• leuB6(Am)-- Suppressed by serU suppressor (supH)
• galK2(Oc)-- is a GAA(glu) to TAA(ochre) mutation, residue 134. Oller et al.1993. Apparently non-suppressible, since all revertants found were at the ochre site.
• mdh-2-- It has been noted that mdh mutants in general are leaky and are distinguished from wild-type alleles by colony size at 24-46 hours on media containing malate (autoclaved and at pH 6.0).
• galK2(Oc) was formerly called gal₂
• thiE1 was formerly called thi-1
• Am = amber(UAG) mutation
• Oc = ochre(UAA) mutation
• strR = streptomycin resist.

References:

• Courtright, J.B., U. Henning 1970. Malate dehydrogenase mutants in Escherichia coli K-12. J.Bacteriol. 102:722-728
• Heard, JT, MA Butler, JN Baptist, TS Matney 1975. Chromosomal location of mutations affecting the electrophoretic mobility of malate dehydrogenase in Escherichia coli K-12. J. Bacteriol. 122(1):329-31.