CGSC Strain#: 6479
Strain Designation: RD22      Source of Strain: R. Deonier
Sex: F' Episome/Plasmid: F203
   Plasmid Markers/Mutations: lac , purE , proC
Chromosomal Markers: Δ(gpt-lac)5, glnX44(AS), λ^-, recA56, relA1, spoT1
Strain Comments:

• Δ(gpt-lac)5-- is the deletion Δ( pro-lac)_X111 of Jacob and originated in the strain Hfr 3000 X111.
• glnX44(AS)-- Mutation is a C to T transition at nucleotide 34 (3rd position of the anticodon)
• recA56-- Amino acid substitution Arg 60 Cys
• spoT1-- In addition to the 6bp insertion, there is a C to T transition resulting in an H257Y substitution.
• glnX44(AS) was formerly called glnV44
• glnX44(AS) was formerly called su⁺_II
• glnX44(AS) was formerly called supE44
• AS = amber(UAG) suppressor

References:

• Deonier, RC, L Mirels 1977. Excision of F plasmid sequences by recombination at directly repeated insertion sequence 2 elements: involvement of recA. Proc. Natl. Acad. Sci. U.S.A. 74(9):3965-9.