CGSC Strain#: 5364
Strain Designation: JP5127      Source of Strain: R.R.B. Russell
Sex: F- Chromosomal Markers: tsx-358, glnX44(AS), galE45(GalS), trp-356, hisG4(Oc), fucP-3, rpsL704(strR), xylA5, mtl-1, ilvC7, argE3(Oc), thiE1, mal358, tfr-3, λ^R
Strain Comments:

• 1. R.R.B. Russell and A. Egan suggest these procedures for use of JP5127 for the transduction of rel:
• 2. The concentration of propanediol in minimal plates must be 0.2% at least. Transduction to growth on propanediol may require 5 days' incubation at 37 degrees C.
• 3. In some genetic backgrounds, there is no apparent cotransduction of fuc-3 with argA or rel.
• 4. It is suggested that as much as 0.1 ml of the undiluted transduction mixture be plated (as well as normal dilutions) as the frequency of transductions may be low.
• glnX44(AS)-- Mutation is a C to T transition at nucleotide 34 (3rd position of the anticodon)
• galE45(GalS)-- is from strain PL5 of Buttin.
• fucP-3-- was formerly called prd-1.
• λ^R-- Lambda resistant, locus unknown.
• glnX44(AS) was formerly called su⁺_II
• glnX44(AS) was formerly called supE44
• glnX44(AS) was formerly called glnV44
• thiE1 was formerly called thi-1
• AS = amber(UAG) suppressor
• GalS = UDPGal accum.toxic if GalKRP+
• Oc = ochre(UAA) mutation
• strR = streptomycin resist.

References:

• Russell, RR 1973. Close linkage of prd and rel genes in Escherichia coli K-12. Mol. Gen. Genet. 124(4):369-70.