CGSC Strain#: 5363
Strain Designation: K1-1-4      Source of Strain: H.L. Kornberg
Sex: Hfr    Point of Origin#: 1   Map Position: 96.80   Direction of Marker Transfer: Clockwise
Chromosomal Markers: tsx-82, gltA9, λ^-, pps-1, relA1, thyA56, spoT1, metB1
Strain Comments:

• thyA56-- Strains with this marker must have their medium supplemented with thymine (25ug/ml). This is true even for rich medium such as LB. Otherwise thymine starvation can lead to thymineless death.
• spoT1-- In addition to the 6bp insertion, there is a C to T transition resulting in an H257Y substitution.

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