CGSC Strain#: 5348
Strain Designation: BB26-36      Source of Strain: R.M. Bell
Other Designations: BB2636
Sex: Hfr    Point of Origin#: 2A   Map Position: 12.20   Direction of Marker Transfer: Counterclockwise
Chromosomal Markers: fhuA22, ΔphoA8, ompF627(T2R), plsX50, fadL701(T2R), relA1, glpR2(glp^c), glpD3, pitA10, spoT1, glpK14(fbR), rrnB-2, plsB26, mcrB1, creC510
Strain Comments:

• This strain is not longer has the G-3-P auxotrophy of its parent strain BB26, because of the loss of fb inhibition of GlpK by Fru-1,6-diP, so it can produce G-3-P from glycerol and grow on minimal plus glycerol plus glucose.
• glpD3-- GlpD loss also results in stasis with gluconeogenic C sources, e.g.,succinate. glpA or glpD mutants should be grown on min.salts+1% glycerol-free casein hydrolysate+1% glucose.
• glpD3-- Loss of G-3-P dehydrogenase (aerobic) results in inability of strains to grow on glycerol or glycerol-3-P as sole carbon source.
• spoT1-- In addition to the 6bp insertion, there is a C to T transition resulting in an H257Y substitution.
• glpK14(fbR)-- : insensitive to catabolite repression. insensitive to feedback inhibition by fructose-1,6-bisP
• rrnB-2-- : loss of spacer loop sequence
• creC510-- The constitutive phenotype is due to an R77P amino acid substitution
• creC510 was formerly called phoM510
• Const = constitutive
• fbR = feedback resistant
• glp^c = constitutive expr.glp regulon
• T2R = T2 Resistant

References:

• Bell, RM 1974. Mutants of Escherichia coli defective in membrane phospholipid synthesis: macromolecular synthesis in an sn-glycerol 3-phosphate acyltransferase Km mutant. J. Bacteriol. 117(3):1065-76.
• McIntyre, TM, BK Chamberlain, RE Webster, RM Bell 1977. Mutants of Escherichia coli defective in membrane phospholipid synthesis. Effects of cessation and reinitiation of phospholipid synthesis on macromolecular synthesis and phospholipid turnover. J. Biol. Chem. 252(13):4487-93.
• Larson, T.J., D.N. Ludtke, R.M. Bell, S. Jackowski 1984. sn-Glycerol-3-phosphate auxotrophy of plsB strains of Escherichia coli: evidence that a second mutation, plsX, is required. J.Bacteriol. 160:711-717