CGSC Strain#: 4969
Strain Designation: PL8-31      Source of Strain: R.C. Greene
Other Designations: EWH154
Sex: F- Chromosomal Markers: thr-1, araC14, leuB6(Am), Δ(gpt-proA)62, lacY1, glnX44(AS), galK2(Oc), λ^-, Rac-0, hisC3, rfbC1, metG87, serA25, metK86, Δ(speC-glcB)63, rpsL25(strR), kdgK51, xylA5, mtl-1, thiE1
Strain Comments:

• leuB6(Am)-- Suppressed by serU suppressor (supH)
• glnX44(AS)-- Mutation is a C to T transition at nucleotide 34 (3rd position of the anticodon)
• galK2(Oc)-- is a GAA(glu) to TAA(ochre) mutation, residue 134. Oller et al.1993. Apparently non-suppressible, since all revertants found were at the ochre site.
• Δ(gpt-proA)62 was formerly called proA2
• galK2(Oc) was formerly called gal₂
• glnX44(AS) was formerly called su⁺_II
• glnX44(AS) was formerly called supE44
• glnX44(AS) was formerly called glnV44
• rfbC1 was formerly called rfbD1
• thiE1 was formerly called thi-1
• Am = amber(UAG) mutation
• AS = amber(UAG) suppressor
• Oc = ochre(UAA) mutation
• strR = streptomycin resist.

References:

• Greene, R.C., J.V. Hunter, E.H. Coch 1973. Properties of metK mutants of Escherichia coli K-12. J.Bacteriol. 115:57-67