CGSC Strain#: 4909
Strain Designation: GN3      Source of Strain: G.N. Godson
Sex: F- Chromosomal Markers: ΔphoA8, adk-3(ts), galK35, λ^-, pyrD34, his-68, fadL701(T2R), thyA25, rpsL118(strR), glpR2(glp^c), glpD3, pitA10, xyl-7, mtlA2, spoT1, glpK4, thiE1
Strain Comments:

• adk-3(ts)-- is a temperature-sensitive mutation.
• galK35-- is Brenner's gal^-_U99 from Hfr 3000 U99 and has been called galK99
• thyA25-- Strains with this marker must have their medium supplemented with thymine (25ug/ml). This is true even for rich medium such as LB. Otherwise thymine starvation can lead to thymineless death.
• glpD3-- GlpD loss also results in stasis with gluconeogenic C sources, e.g.,succinate. glpA or glpD mutants should be grown on min.salts+1% glycerol-free casein hydrolysate+1% glucose.
• glpD3-- Loss of G-3-P dehydrogenase (aerobic) results in inability of strains to grow on glycerol or glycerol-3-P as sole carbon source.
• spoT1-- In addition to the 6bp insertion, there is a C to T transition resulting in an H257Y substitution.
• thiE1 was formerly called thi-1
• xyl-7 was formerly called xylA7
• glp^c = constitutive expr.glp regulon
• strR = streptomycin resist.
• T2R = T2 Resistant
• ts = Temperature Sensitive

References:

• Cronan, J.E., G.N. Godson 1972. Mutants of Escherichia coli with temperature-sensitive lesions in membrane phospholipid synthesis: genetic analysis of glycerol-3-phosphate acyltransferase mutants. Mol.Gen.Genet. 116:199-210
• Godson, GN 1973. Isolation and partial characterization of temperature-sensitive mutants in ten loci that affect cell membrane synthesis in Escherichia coli: isolation and genetic sorting. J. Bacteriol. 113(2):813-24.
• Ray, TK, JE Cronan, GN Godson 1976. Specific inhibition of phospholipid synthesis in plsA mutants of Escherichia coli. J. Bacteriol. 125(1):136-41.