CGSC Strain#: 4866
Strain Designation: JC411-5-2      Source of Strain: J. Gerhart
Sex: F' Episome/Plasmid: F20
   Plasmid Markers/Mutations:
Chromosomal Markers: leuB6(Am), fhuA2::IS2, glnX44(AS), gal-6, λ^-, pyrF92, hisG1, argG6, rpsL104, malT1(λ^R), xyl-7, mtlA2, metB1
Strain Comments:

• This strain should be maintained on medium containing leu and his and lacking arg and met.
• leuB6(Am)-- Suppressed by serU suppressor (supH)
• glnX44(AS)-- Mutation is a C to T transition at nucleotide 34 (3rd position of the anticodon)
• hisG1-- This mutation has been reported as either a frameshift or a 36 nt deletion
• fhuA2::IS2 was formerly called fhuA2
• glnX44(AS) was formerly called su⁺_II
• glnX44(AS) was formerly called supE44
• glnX44(AS) was formerly called glnV44
• xyl-7 was formerly called xylA7
• = strep-resistant
• ::IS2 = Insertion of IS2
• Am = amber(UAG) mutation
• AS = amber(UAG) suppressor
• LamR = Lambda-resistant

References:

• Gerhart, JC, H Holoubek 1967. The purification of aspartate transcarbamylase of Escherichia coli and separation of its protein subunits. J. Biol. Chem. 242(12):2886-92.