CGSC Strain#: 4826
Strain Designation: H12      Source of Strain: A. Garen
Sex: Hfr    Point of Origin#: 2A   Map Position: 12.20   Direction of Marker Transfer: Counterclockwise
Chromosomal Markers: garB10, fhuA22, phoA5(Am), ompF627(T2R), fadL701(T2R), relA1, pitA10, spoT1, phoT-35, rrnB-2, mcrB1, creC510
Strain Comments:

• phoA5(Am)-- is Garen's phoAH12.
• spoT1-- In addition to the 6bp insertion, there is a C to T transition resulting in an H257Y substitution.
• phoT-35-- is a phoS or phoT mutation.
• rrnB-2-- : loss of spacer loop sequence
• creC510-- The constitutive phenotype is due to an R77P amino acid substitution
• creC510 was formerly called phoM510
• Am = amber(UAG) mutation
• Const = constitutive
• T2R = T2 Resistant

References:

• Garen, A., S. Garen, R. Wilhelm 1965. Suppressor genes for nonsense mutations. I. the Su-1, Su-2, and Su-3 genes of Escherichia coli. J.Mol.Biol. 14:167-187
• Weigert, M.G., A. Garen 1965. Amino acid substitutions resulting from suppression of nonsense mutations. J.Mol.Biol. 12:448-455
• Gallucci, E., A. Garen 1966. Suppressor genes for nonsense mutations. II. The Su-4 and Su-5 suppressor genes of E. coli. J.Mol.Biol. 15:193-200