CGSC Strain#: 12136
Strain Designation: RK9118      Source of Strain: R.J. Kadner
Sex: F- Chromosomal Markers: [araD139]_B/r, Δ(argF-lac)169, non-9, gyrA219(NalR), relA1, rpsL150(strR), Δ(uhpT-ilvB)2095, zig-621::Tn10 uhpA⁺B⁺C^-T⁺, polA1(Am), thiE1
Strain Comments:

• [araD139]_B/r-- The thr-leu region was transduced from an E. coli B/r strain (SB3118) in early steps of strain derivation; ara or in some strains ara-leu or thr-leu are from B.
• Δ(argF-lac)169-- extends from mmuP through orfs preceding argF, through lac to mhpD, literally Δ(mmuP-mhpD)169. (Peters et al. 2003 JB 185:2017)
• Δ(argF-lac)169-- from strain Hfr3000 U169 was initially called ΔlacU169 and described as a lacZY mutation until found to include argF and lacI.
• rpsL150(strR)-- T to C transition mutation resulting in a K43R amino acid substitution.
• zig-621::Tn10 uhpA⁺B⁺C^-T⁺-- uhpC 91::8 has an 8aa insertion after residue 91 resulting in a uhpC negative phenotype
• Δ(uhpT-ilvB)2095 was formerly called Δ(ilvBN-uhpABCT')2095
• thiE1 was formerly called thi-1
• zig-621::Tn10 uhpA⁺B⁺C^-T⁺ was formerly called zig-621::Tn10 uhpA⁺B⁺C 91::8 (Neg)T⁺
• = tetracycline resistant
• Am = amber(UAG) mutation
• NalR = Nalidix.acidResist.
• strR = streptomycin resist.

References:

• Shearer, G, JC Lee, JA Koo, DH Kohl 2005. Quantitative estimation of channeling from early glycolytic intermediates to CO in intact Escherichia coli. FEBS J. 272(13):3260-9.