CGSC Strain#: 4812
Strain Designation: T850      Source of Strain: T. Takano
Sex: F- Chromosomal Markers: fhuA2::IS2, proA44, lacY1, glnX44(AS), gal-6, λ^-, hisG1, malT1(λ^R), xyl-7, mtlA2, ΔargH1, rplL9(L?), thiE1, groE-1
Strain Comments:

• glnX44(AS)-- Mutation is a C to T transition at nucleotide 34 (3rd position of the anticodon)
• hisG1-- This mutation has been reported as either a frameshift or a 36 nt deletion
• groE-1-- : The strain is TS on minimal, not on Luria
• groE-1-- The (gro-purA region of this strain comes from a derivative of E. coli B.
• fhuA2::IS2 was formerly called fhuA2
• glnX44(AS) was formerly called su⁺_II
• glnX44(AS) was formerly called supE44
• glnX44(AS) was formerly called glnV44
• thiE1 was formerly called thi-1
• xyl-7 was formerly called xylA7
• ::IS2 = Insertion of IS2
• AS = amber(UAG) suppressor
• L? = assignment uncertain
• LamR = Lambda-resistant

References:

• Takano, T, T Kakefuda 1972. Involvement of a bacterial factor in morphogenesis of bacteriophage capsid. Nature New Biol. 239(89):34-7.
• Govezensky, D, T Greener, G Segal, A Zamir 1991. Involvement of GroEL in nif gene regulation and nitrogenase assembly. J. Bacteriol. 173(20):6339-46.
• Greener, T., D. Govezensky, A. Zamir 1993. A novel multicopy suppressor of a groEL mutation includes two nested open reading frames transcribed from different promoters. EMBO J. 12:889-896