CGSC Strain#: 4548
Strain Designation: AT998      Source of Strain: A.L. Taylor
Sex: Hfr    Point of Origin#: 45   Map Position: 65.00   Direction of Marker Transfer: Counterclockwise
Chromosomal Markers: λ^-, e14-, dapA16, relA1, spoT1, thiE1
Strain Comments:

• 1.This strain can be maintained on the standard rich media only if the medium is supplemented with 40-50 ug/ml of DL-diaminopimelic acid.
• 2.The dapA mutation leads to a requirement for isoleucine and valine also.The strain may carry a temperature-sensitive mutation.
• e14--- : Loss of the e14 element makes the strain McrA^-.
• dapA16-- dapA16 causes a requirement for ILV and the strain grows poorly.
• spoT1-- In addition to the 6bp insertion, there is a C to T transition resulting in an H257Y substitution.
• thiE1 was formerly called thi-1

References:

• Bukhari, A.I., A.L. Taylor 1971. Genetic analysis of diaminopimelic acid- and lysine-requiring mutants of Escherichia coli. J.Bacteriol. 105:844-854