CGSC Strain#: 4524
Strain Designation: AT2699      Source of Strain: A.L. Taylor
Other Designations: EWH159
Sex: F- Chromosomal Markers: lacY1, tsx-1, glnX44(AS), gal-6, λ^-, hisG1, thyA3, metC69, argG6, rpsL8 or rpsL104 or rpsL17, malT1(λ^R)
Strain Comments:

• glnX44(AS)-- Mutation is a C to T transition at nucleotide 34 (3rd position of the anticodon)
• hisG1-- This mutation has been reported as either a frameshift or a 36 nt deletion
• thyA3-- Strains with this marker must have their medium supplemented with thymine (25ug/ml). This is true even for rich medium such as LB. Otherwise thymine starvation can lead to thymineless death.
• metC69-- was called metC58 by A.L. Taylor.
• glnX44(AS) was formerly called glnV44
• glnX44(AS) was formerly called su⁺_II
• glnX44(AS) was formerly called supE44
• = strep-resistant
• AS = amber(UAG) suppressor
• LamR = Lambda-resistant

References: