CGSC Strain#: 4523
Strain Designation: AT2696      Source of Strain: A.L. Taylor
Sex: F- Chromosomal Markers: glnX44(AS), λ^-, hisG1, thyA3, lysA22, rpsL104, malT1(λ^R), xyl-7, mtlA2, metB1
Strain Comments:

• glnX44(AS)-- Mutation is a C to T transition at nucleotide 34 (3rd position of the anticodon)
• hisG1-- This mutation has been reported as either a frameshift or a 36 nt deletion
• thyA3-- Strains with this marker must have their medium supplemented with thymine (25ug/ml). This is true even for rich medium such as LB. Otherwise thymine starvation can lead to thymineless death.
• lysA22-- is Taylor's lysA10 which he briefly called lysB10 in 1971.
• glnX44(AS) was formerly called su⁺_II
• glnX44(AS) was formerly called supE44
• glnX44(AS) was formerly called glnV44
• xyl-7 was formerly called xylA7
• = strep-resistant
• AS = amber(UAG) suppressor
• LamR = Lambda-resistant

References:

• Taylor, A.L., C.D. Trotter 1967. Revised linkage map of Escherichia coli. Bacteriol.Rev. 31:332-353
• Taylor, A.L. 1970. Current linkage map of Escherichia coli. Bacteriol.Rev. 34:155-175