CGSC Strain#: 4508
Strain Designation: AT2459      Source of Strain: A.L. Taylor
Sex: Hfr    Point of Origin#: 1   Map Position: 96.80   Direction of Marker Transfer: Clockwise
Chromosomal Markers: λ^-, e14-, relA1, spoT1, thiE1, serB22
Strain Comments:

• This strain grows poorly on serine and hence is best used as a recipient for Ser⁺ selection. It should transfer serB early but it may have become an F⁺.
• e14--- : Loss of the e14 element makes the strain McrA^-.
• spoT1-- In addition to the 6bp insertion, there is a C to T transition resulting in an H257Y substitution.
• serB22-- : strain grows poorly on serine,
• thiE1 was formerly called thi-1

References:

• Taylor, A.L., C.D. Trotter 1967. Revised linkage map of Escherichia coli. Bacteriol.Rev. 31:332-353