CGSC Strain#: 4500
Strain Designation: Hfr 3000 YA149      Source of Strain: W. Jacobs
Other Designations: AT2242
Sex: Hfr    Point of Origin#: 1   Map Position: 96.80   Direction of Marker Transfer: Clockwise
Chromosomal Markers: λ^-, e14-, pyrF40, relA1, spoT1, thiE1
Strain Comments:

• e14--- : Loss of the e14 element makes the strain McrA^-.
• spoT1-- In addition to the 6bp insertion, there is a C to T transition resulting in an H257Y substitution.
• thiE1 was formerly called thi-1

References:

• Beckwith, J.R., A.B. Pardee, R. Austrian, F. Jacob 1962. Coordination of the synthesis of the enzymes in the pyrimidine pathway of E. coli. J.Mol.Biol. 5:618-634