CGSC Strain#: 4253
Strain Designation: KL703      Source of Strain: M.C. Cappello
Sex: F' Episome/Plasmid: F126
   Plasmid Markers/Mutations:
Chromosomal Markers: galK35, λ^-, pyrD34, trpC45, his-68, recA1, rpsL118(strR), malT1(λ^R), xyl-7, mtlA2, thiE1
Strain Comments:

• This strain should be maintained on medium with galactose as carbon source, containing thi, his and lacking ura, trp. The strain shows a Gal^- phenotype on MacConkey-Gal medium.
• galK35-- is Brenner's gal^-_U99 from Hfr 3000 U99 and has been called galK99
• recA1-- : Missense mutation, altered isoelectric point. Sequenced: G to A for Nuc. 720 (Gly 160 Asp).
• thiE1 was formerly called thi-1
• xyl-7 was formerly called xylA7
• LamR = Lambda-resistant
• strR = streptomycin resist.

References:

• Low, KB 1972. Escherichia coli K-12 F-prime factors, old and new. 36(4):587-607.