CGSC Strain#: 4249
Strain Designation: KL721      
Source of Strain: M.C. Cappello
Other Designations: KLF3
Sex: F' 
Episome/Plasmid: F103
   Plasmid Markers/Mutations:  metG  Chromosomal Markers: leuB6(Am)
, 
fhuA2::IS2, 
lacY1, 
tsx-1, 
glnX44(AS)
, 
gal-6, 
λ-, 
trp-31, 
hisG1, 
argG6, 
rpsL104, 
malT1(λ
R)
, 
xyl-7, 
mtlA2, 
metB1Strain Comments: - This strain must be grown on medium containing arg, leu, met, try and lacking his.
- leuB6(Am)-- Suppressed by serU suppressor (supH)                                                                                                                                                                                                                          
- glnX44(AS)-- Mutation is a C to T transition at nucleotide 34 (3rd position of the anticodon)
- hisG1-- This mutation has been reported as either a frameshift or a 36 nt deletion 
- fhuA2::IS2 was formerly called fhuA2
- glnX44(AS) was formerly called su+II
- glnX44(AS) was formerly called supE44
- glnX44(AS) was formerly called glnV44
- xyl-7 was formerly called xylA7
-  = strep-resistant
- ::IS2 = Insertion of IS2
- Am = amber(UAG) mutation
- AS = amber(UAG) suppressor
- LamR = Lambda-resistant
References: