CGSC Strain#: 2874
Strain Designation: AB2874      Source of Strain: A.J. Pittard
Sex: F- Chromosomal Markers: Δ(gpt-proA)62, lacY1, tsx-358, glnX44(AS), galK2(Oc), hisG4(Oc), aroF363, xylA5, mtl-1, ilvC7, argE3(Oc), thiE1, tfr-3
Strain Comments:

• This strain can be grown on Phe, Tyr, Try plus shikimic acid at 10^-6M.
• glnX44(AS)-- Mutation is a C to T transition at nucleotide 34 (3rd position of the anticodon)
• galK2(Oc)-- is a GAA(glu) to TAA(ochre) mutation, residue 134. Oller et al.1993. Apparently non-suppressible, since all revertants found were at the ochre site.
• Δ(gpt-proA)62 was formerly called proA2
• galK2(Oc) was formerly called gal₂
• glnX44(AS) was formerly called su⁺_II
• glnX44(AS) was formerly called supE44
• glnX44(AS) was formerly called glnV44
• thiE1 was formerly called thi-1
• AS = amber(UAG) suppressor
• Oc = ochre(UAA) mutation

References:

• Wallace, BJ, J Pittard 1967. Genetic and biochemical analysis of the isoenzymes concerned in the first reaction of aromatic biosynthesis in Escherichia coli. J. Bacteriol. 93(1):237-44.