CGSC Strain#: 4451
Strain Designation: RBH066      Source of Strain: R.B. Helling
Other Designations: ``AB259, icd-3"
Sex: Hfr    Point of Origin#: 1   Map Position: 96.80   Direction of Marker Transfer: Clockwise
Chromosomal Markers: λ^-, icd-3, e14-, relA1, spoT1, thiE1
Strain Comments:

• e14--- : Loss of the e14 element makes the strain McrA^-.
• spoT1-- In addition to the 6bp insertion, there is a C to T transition resulting in an H257Y substitution.
• thiE1 was formerly called thi-1

References:

• Helling, R.B., J. Kukora 1971. Nalidixic acid-resistant mutants of Escherichia coli deficient in isocitrate dehydrogenase. J.Bacteriol. 105:1224-1226