CGSC Strain#: 6965
Strain Designation: LS6036      Source of Strain: W.D. Nunn
Sex: F- Chromosomal Markers: leuB6(Am), λ^-, thyA47, rpsL153(strR), zjh-2007::Tn10, prpA12, dnaC1(ts), deoC3
Strain Comments:

• The positions of the prp locus and the Tn 10 insertion are not accurately determined.
• leuB6(Am)-- Suppressed by serU suppressor (supH)
• thyA47-- Strains with this marker must have their medium supplemented with thymine (25ug/ml). This is true even for rich medium such as LB. Otherwise thymine starvation can lead to thymineless death.
• = tetracycline resistant
• Am = amber(UAG) mutation
• strR = streptomycin resist.
• ts = Temperature Sensitive

References:

• Spratt, B.G., C.L. Ginsburgh, W.D. Nunn 1981. Isolation and genetic characterization of Escherichia coli mutants defective in propionate metabolism. J.Bacteriol. 146:1166-1169