CGSC Strain#: 6960
Strain Designation: TL212      Source of Strain: T.J. Larson
Sex: F- Chromosomal Markers: [araD139]_B/r, Δ(argF-lac)169, λ^-, zce-726::Tn10, flhD5301, Δ(fruK-yeiR)725(fruA25), relA1, rpsL150(strR), malT101(Const), ΔmalE444, deoC1
Strain Comments:

• The thr-ara region was transduced in from E. coli B.
• [araD139]_B/r-- The thr-leu region was transduced from an E. coli B/r strain (SB3118) in early steps of strain derivation; ara or in some strains ara-leu or thr-leu are from B.
• Δ(argF-lac)169-- extends from mmuP through orfs preceding argF, through lac to mhpD, literally Δ(mmuP-mhpD)169. (Peters et al. 2003 JB 185:2017)
• Δ(argF-lac)169-- from strain Hfr3000 U169 was initially called ΔlacU169 and described as a lacZY mutation until found to include argF and lacI.
• zce-726::Tn10-- cotransduces 9% with pyrC and 85% with ptsG (and 5% with csgD). PCR analysis (Nichols et al.1998) places this insertion in orf yceG, at nucleotide 1,154,216 (24.88 min.).
• flhD5301-- flagellar defect shown in Komeda and Iino
• Δ(fruK-yeiR)725(fruA25)-- : this fruA25 mutation deletes 29 codons of fruK, fruB and fruBKAp promoter to orf yeiR Peters et al. J.Bact.185:2017.
• rpsL150(strR)-- T to C transition mutation resulting in a K43R amino acid substitution.
• malT101(Const)-- In the paper cited malT101 is designated malT^C-1.
• Δ(fruK-yeiR)725(fruA25) was formerly called fruA25
• = tetracycline resistant
• Const = constitutive
• fruA25 = former designation
• strR = streptomycin resist.

References:

• Larson, T.J., D.N. Ludtke, R.M. Bell, S. Jackowski 1984. sn-Glycerol-3-phosphate auxotrophy of plsB strains of Escherichia coli: evidence that a second mutation, plsX, is required. J.Bacteriol. 160:711-717