CGSC Strain#: 6776
Strain Designation: YYC188      Source of Strain: J.E. Cronan
Sex: Hfr    Point of Origin#: 2A   Map Position: 12.20   Direction of Marker Transfer: Counterclockwise
Chromosomal Markers: secA214(aziR), Δ(aroP-aceF)73, fhuA54, lacZ608(Am), LAMcI857(ts), zbj-927::Tn10, poxB11, pflB1, tonB54, pps-4, rpsL104, thiE1
Strain Comments:

• This strain must be grown at a temperature below 33 degrees C.
• lacZ608(Am)-- is an amber mutation (Newton et al. 1965 J. Mol. Biol. 14:290), aka lacZ^-NG608 by Zinder, in segment 10 of the lacZ gene (Zipser et al. 1970).
• zbj-927::Tn10-- cotransduces ~72% with cmlA and ~ 71% with poxB.
• secA214(aziR) was formerly called secA14
• secA214(aziR) was formerly called azi-14
• thiE1 was formerly called thi-1
• = strep-resistant
• = tetracycline resistant
• Am = amber(UAG) mutation
• aziR = azide resistance
• ts = Temperature Sensitive

References:

• Chang, Y.-Y., J.E. Cronan 1983. Genetic and biochemical analyses of Escherichia coli strains having a mutation in the structural gene (poxB) for pyruvate oxidase. J.Bacteriol. 154:756-762