CGSC Strain#: 6774
Strain Designation: YYC7      Source of Strain: J.E. Cronan
Sex: Hfr    Point of Origin#: 2A   Map Position: 12.20   Direction of Marker Transfer: Counterclockwise
Chromosomal Markers: secA214(aziR), Δ(aroP-aceF)73, fhuA54, lacZ608(Am), LAMcI857(ts), tonB54, thiE1, poxA1
Strain Comments:

• This strain must be grown at a temperature below 33 degrees C.
• lacZ608(Am)-- is an amber mutation (Newton et al. 1965 J. Mol. Biol. 14:290), aka lacZ^-NG608 by Zinder, in segment 10 of the lacZ gene (Zipser et al. 1970).
• secA214(aziR) was formerly called azi-14
• secA214(aziR) was formerly called secA14
• thiE1 was formerly called thi-1
• Am = amber(UAG) mutation
• aziR = azide resistance
• ts = Temperature Sensitive

References:

• Chang, Y.-Y., J.E. Cronan 1982. Mapping nonselectable genes of Escherichia coli by using transposon Tn10 location of a gene affecting pyruvate oxidase. J.Bacteriol. 151:1279-1289
• Chang, Y.-Y., J.E. Cronan 1983. Genetic and biochemical analyses of Escherichia coli strains having a mutation in the structural gene (poxB) for pyruvate oxidase. J.Bacteriol. 154:756-762