CGSC Strain#: 6540
Strain Designation: LCB358      Source of Strain: J. Puig
Sex: F- Chromosomal Markers: thr-1, leuB6(Am), fhuA2::IS2, pro-33, lacY1, purE43, glnX44(AS), gal-6, λ^-, moaA15, hisG1, rfbC1, galP63, Δ(gltB-gltF)500, malT1(λ^R), xyl-7, mtlA2, ΔargH1, rplL9(L?), thiE1
Strain Comments:

• leuB6(Am)-- Suppressed by serU suppressor (supH)
• glnX44(AS)-- Mutation is a C to T transition at nucleotide 34 (3rd position of the anticodon)
• hisG1-- This mutation has been reported as either a frameshift or a 36 nt deletion
• Δ(gltB-gltF)500-- :Glt^- phenotype in gltB mutant requires presence of a gdhA mutation as well. 0.2% aspartate is a better N-source than glutamate because of uptake(Pahel et al).
• Δ(gltB-gltF)500 was formerly called aspB24
• Δ(gltB-gltF)500 was formerly called aspB21
• Δ(gltB-gltF)500 was formerly called gltB31
• fhuA2::IS2 was formerly called fhuA2
• glnX44(AS) was formerly called su⁺_II
• glnX44(AS) was formerly called supE44
• glnX44(AS) was formerly called glnV44
• rfbC1 was formerly called rfbD1
• thiE1 was formerly called thi-1
• xyl-7 was formerly called xylA7
• ::IS2 = Insertion of IS2
• Am = amber(UAG) mutation
• AS = amber(UAG) suppressor
• L? = assignment uncertain
• LamR = Lambda-resistant

References:

• Puig, J., E. Azoulay 1967. Etude genetique et biochimique des mutants resistant au ClO3-(genes chlA,chl,chlC). Compt.Rend.Acad.Sci.Paris 264:1916-1918
• Puig, J., E. Azoulay, J. Gendre, E. Richard 1969. Etude genetique des mutants de la region chlA chez l'Escherichia coli. Compt.Rend.Acad.Sci.Paris 268:183-184